5M6U
HUMAN PI3KDELTA IN COMPLEX WITH LASW1579
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-03-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.493, 113.212, 144.321 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.080 - 2.850 |
| R-factor | 0.2569 |
| Rwork | 0.256 |
| R-free | 0.30890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wxr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.055 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.080 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.040 | 0.700 |
| Number of reflections | 36112 | |
| <I/σ(I)> | 7.7 | 1.1 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 6.5 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | NULL |
