5M5G

Crystal structure of the Chaetomium Thermophilum polycomb repressive complex 2 (PRC2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	93
Detector technology	CCD
Collection date	2015-02-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.063
Spacegroup name	P 1 21 1
Unit cell lengths	81.554, 74.612, 128.508
Unit cell angles	90.00, 107.70, 90.00

Refinement procedure

Resolution	45.840 - 2.270
R-factor	0.17343
Rwork	0.171
R-free	0.22146
RMSD bond length	0.016
RMSD bond angle	1.720
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.840
High resolution limit [Å]	2.270
Number of reflections	58693
<I/σ(I)>	1.81
Completeness [%]	90.7
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	15% PEG4000, 175mM ammonium citrate, pH 7.0
1	VAPOR DIFFUSION, HANGING DROP	7	293	15% PEG4000, 175mM ammonium citrate, pH 7.0