5M5B
Crystal structure of Zika virus NS5 methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.98009 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.550, 64.140, 72.040 |
| Unit cell angles | 113.05, 97.78, 92.00 |
Refinement procedure
| Resolution | 65.410 - 2.010 |
| R-factor | 0.162 |
| Rwork | 0.160 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3p97 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.960 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.410 | 2.022 |
| High resolution limit [Å] | 2.010 | 2.015 |
| Rmerge | 0.132 | 0.359 |
| Rmeas | 0.157 | 0.454 |
| Rpim | 0.083 | 0.272 |
| Number of reflections | 40055 | |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 150 mM sodium citrate 1.5 M ammonium sulfate |
