5M34
Structure of cobinamide-bound BtuF mutant W66Y, the periplasmic vitamin B12 binding protein in E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 123 |
| Detector technology | PIXEL |
| Collection date | 2014-12-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97794 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.830, 90.800, 51.070 |
| Unit cell angles | 90.00, 111.25, 90.00 |
Refinement procedure
| Resolution | 29.480 - 1.600 |
| R-factor | 0.19327 |
| Rwork | 0.191 |
| R-free | 0.23334 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5m29 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.609 |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (1.10.1-2155) |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.480 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 73585 |
| <I/σ(I)> | 9.77 |
| Completeness [%] | 93.2 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | PEG3350 HEPES pH 7 Tryptone |
