5M29
Structure of cobinamide-bound BtuF, the periplasmic vitamin B12 binding protein in E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 123 |
| Detector technology | PIXEL |
| Collection date | 2013-09-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.00001 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.150, 90.560, 50.840 |
| Unit cell angles | 90.00, 110.90, 90.00 |
Refinement procedure
| Resolution | 29.390 - 1.500 |
| R-factor | 0.18073 |
| Rwork | 0.179 |
| R-free | 0.21296 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n2z |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.228 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (1.8.2-1309) |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.390 | 1.690 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.059 | 0.849 |
| Number of reflections | 89615 | |
| <I/σ(I)> | 10.16 | |
| Completeness [%] | 96.0 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | HEPES sodium pH 7.0 PEG3350 Tryptone |
