5M13

Synthetic nanobody in complex with MBP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-16
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00004
Spacegroup name	P 21 21 21
Unit cell lengths	58.298, 82.789, 102.583
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.666 - 1.372
R-factor	0.1691
Rwork	0.168
R-free	0.18600
RMSD bond length	0.005
RMSD bond angle	0.750
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.372
Number of reflections	196280
<I/σ(I)>	10.04
Completeness [%]	97.8
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	100 mM Tris pH 8.5, 25 % PEG3350