5M05
Chicken smooth muscle myosin motor domain co-crystallized with the specific CK-571 inhibitor, MgADP form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.980110 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 73.190, 202.970, 67.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.680 - 2.675 |
| R-factor | 0.1876 |
| Rwork | 0.185 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1br1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.243 |
| Data reduction software | XDS (10.9.4) |
| Data scaling software | XSCALE (10.90.4) |
| Phasing software | PHASER (6.4.0) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.680 | 2.770 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmerge | 0.100 | 0.800 |
| Number of reflections | 29000 | |
| <I/σ(I)> | 14.23 | 1.97 |
| Completeness [%] | 99.0 | 91 |
| Redundancy | 7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 277 | Peg8K, 50 mM Bicine pH 8.2, 10% DMSO |
