5LXI
GABARAP-L1 ATG4B LIR Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.888, 72.377, 30.620 |
| Unit cell angles | 90.00, 99.21, 90.00 |
Refinement procedure
| Resolution | 31.207 - 1.440 |
| R-factor | 0.1987 |
| Rwork | 0.197 |
| R-free | 0.22990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r2q |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.159 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.470 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.350 | |
| Number of reflections | 46217 | |
| <I/σ(I)> | 10.1 | 1.3 |
| Completeness [%] | 96.0 | 93.7 |
| Redundancy | 3.1 | 3 |
| CC(1/2) | 0.998 | 0.740 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MgCl2, 24.6 % PEG 400, 29.5 % PEG 8000, 0.1M TRIS 8.5 |
