5LXG

Revised crystal structure of the human adiponectin receptor 1 in an open conformation

Replaces:  3WXV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-23
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	92.310, 194.110, 74.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.660 - 2.730
R-factor	0.225
Rwork	0.225
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3WXV
RMSD bond length	0.008
RMSD bond angle	0.970
Data reduction software	HKL-2000
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	97.060
High resolution limit [Å]	2.730
Rmerge	0.171
Number of reflections	17815
<I/σ(I)>	7.934
Completeness [%]	99.7
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	8	293	100mM Bicine, 100mM MgCl2, 30% PEG400, pH 8.0, Lipidic mesophase method, temperature 293K