5LXB
Crystal structure of a mutant binding protein (5HTBP-AChBP) in complex with palonosetron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2004-12-12 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.215, 137.051, 131.249 |
| Unit cell angles | 90.00, 93.78, 90.00 |
Refinement procedure
| Resolution | 130.960 - 2.340 |
| R-factor | 0.2192 |
| Rwork | 0.218 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yme |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.819 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0157) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 130.960 | 2.470 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.845 | |
| Number of reflections | 106052 | |
| <I/σ(I)> | 8.9 | 1.6 |
| Completeness [%] | 99.2 | 94.5 |
| Redundancy | 4.5 | 4.4 |
| CC(1/2) | 0.598 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM sodium 265 citrate, 100 mM bis tris propane pH 8.5, and 20% (w/v) PEG3350. |
