5LWF
Structure of a single domain camelid antibody fragment cAb-G10S in complex with the BlaP beta-lactamase from Bacillus licheniformis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.278, 121.691, 137.029 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.500 - 2.560 |
| R-factor | 0.217 |
| Rwork | 0.215 |
| R-free | 0.24200 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.459 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 54511 |
| <I/σ(I)> | 9.72 |
| Completeness [%] | 98.9 |
| Redundancy | 3.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Mg Acetate, 0.1 M NaCacodylate pH 6.5, 20% PEG8000 |
