5LW0
Oryza sativa APL macrodomain in complex with ADP-ribose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.496, 91.318, 62.882 |
| Unit cell angles | 90.00, 103.32, 90.00 |
Refinement procedure
| Resolution | 56.310 - 1.650 |
| R-factor | 0.1564 |
| Rwork | 0.155 |
| R-free | 0.18727 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lw6 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.564 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.310 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.080 | 0.406 |
| Number of reflections | 47191 | |
| <I/σ(I)> | 12.8 | 3.2 |
| Completeness [%] | 97.4 | 77.7 |
| Redundancy | 6.7 | 5.2 |
| CC(1/2) | 0.996 | 0.875 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 25% PEG 1500 + 0.1 M SPG buffer (succinic acid, sodium phosphate monobasic monohydrate and glycine) |
