5LVT
Structure of HU protein from Lactococcus lactis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-03 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.984 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 47.774, 99.002, 168.281 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.030 - 2.100 |
R-factor | 0.186 |
Rwork | 0.185 |
R-free | 0.20800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mul |
RMSD bond length | 0.010 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.030 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.076 | 0.895 |
Number of reflections | 22969 | |
<I/σ(I)> | 11.4 | 1.9 |
Completeness [%] | 97.3 | 99.5 |
Redundancy | 5.1 | 5.1 |
CC(1/2) | 0.998 | 0.593 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 100mM Na acetate, 2M ammonium sulfate |