5LV4
Crystal structure of mouse PRMT6 in complex with inhibitor LH1236
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97779 |
| Spacegroup name | I 41 |
| Unit cell lengths | 79.851, 79.851, 119.738 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.080 - 1.660 |
| R-factor | 0.16 |
| Rwork | 0.159 |
| R-free | 0.18820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c07 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.079 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10pre_2104: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.080 | 1.690 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.142 | 1.508 |
| Number of reflections | 44207 | |
| <I/σ(I)> | 7.7 | 1 |
| Completeness [%] | 99.7 | 94.5 |
| Redundancy | 7.2 | 5.5 |
| CC(1/2) | 0.996 | 0.474 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | HEPES NaOH pH 8.0 100 mM PEG 6000 10 % MgCl2 200 mM |
