5LV0

Structure of Human Neurolysin (E475Q) in complex with amyloid-beta 35-40 peptide product

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976
Spacegroup name	P 43 21 2
Unit cell lengths	131.341, 131.341, 195.979
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	109.110 - 2.700
R-factor	0.21203
Rwork	0.210
R-free	0.26049
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i1i
RMSD bond length	0.007
RMSD bond angle	1.102
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.15)
Phasing software	PHASER (2.5.7)
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	109.110	2.790
High resolution limit [Å]	2.700	2.700
Rmerge	0.093	0.600
Number of reflections	47196
<I/σ(I)>	13.7	3.2
Completeness [%]	99.2	99.6
Redundancy	8.1	8.4
CC(1/2)	0.985	0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	0.1M Bis-Tris-propane, 11% PEG 3350, 10% glycerol, 0.2M potassium thiocyanate