5LUV

Short LOV protein W619_1 in apo-state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	I 41
Unit cell lengths	109.538, 109.538, 95.209
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.559 - 2.500
R-factor	0.2129
Rwork	0.211
R-free	0.25280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j3w
RMSD bond length	0.009
RMSD bond angle	1.015
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.600	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.101	1.908
Number of reflections	19498
<I/σ(I)>	12.3	1.1
Completeness [%]	99.9	100
Redundancy	5.7	5.9
CC(1/2)	0.998	0.336

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		287	1M NH4SO4, 0.1M MES