5LUP
Structures of DHBN domain of human BLM helicase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.7 |
Spacegroup name | P 62 |
Unit cell lengths | 132.795, 132.795, 64.984 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.470 - 2.032 |
R-factor | 0.2129 |
Rwork | 0.210 |
R-free | 0.26260 |
Structure solution method | SIRAS |
RMSD bond length | 0.007 |
RMSD bond angle | 0.952 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.470 | |
High resolution limit [Å] | 2.032 | 2.105 |
Rmerge | 0.074 | 0.534 |
Number of reflections | 41409 | |
<I/σ(I)> | 18.34 | 1.84 |
Completeness [%] | 98.0 | 87 |
Redundancy | 8.2 | 3.2 |
CC(1/2) | 0.999 | 0.767 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | Tris-HCl 0.1M PH8.5 PEG1500 2% Glycerol 16% |