5LTZ
GmhA_mutant Q175E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.550, 84.473, 126.682 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.250 - 1.670 |
| R-factor | 0.2095 |
| Rwork | 0.207 |
| R-free | 0.24470 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.059 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.870 |
| High resolution limit [Å] | 1.670 |
| Number of reflections | 90441 |
| <I/σ(I)> | 11.8 |
| Completeness [%] | 98.4 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 277.15 | B. pseudomallei GmhA at 10 mg per mL was mixed with an equal volume of crystallization solution: 0.1 M Na Acetate, pH 4.6, 8% PEG 4000 |
