5LTY

Homeobox transcription factor CDX2 bound to methylated DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9724
Spacegroup name	I 1 2 1
Unit cell lengths	68.882, 46.613, 120.428
Unit cell angles	90.00, 98.46, 90.00

Refinement procedure

Resolution	63.290 - 2.660
R-factor	0.2039
Rwork	0.202
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eea
RMSD bond length	0.017
RMSD bond angle	2.161
Data reduction software	XDS
Data scaling software	Aimless (0.5.25)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.290	2.790
High resolution limit [Å]	2.660	2.660
Rmerge	0.043	0.637
Number of reflections	10979
<I/σ(I)>	15	1.7
Completeness [%]	98.5	96.7
Redundancy	3.3	3.1
CC(1/2)	0.999	0.647

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	PEG 5000, potassium chloride, magnesium chloride, PEG 400,Tris buffer