5LTY
Homeobox transcription factor CDX2 bound to methylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9724 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 68.882, 46.613, 120.428 |
| Unit cell angles | 90.00, 98.46, 90.00 |
Refinement procedure
| Resolution | 63.290 - 2.660 |
| R-factor | 0.2039 |
| Rwork | 0.202 |
| R-free | 0.24220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5eea |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.161 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.25) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.290 | 2.790 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Rmerge | 0.043 | 0.637 |
| Number of reflections | 10979 | |
| <I/σ(I)> | 15 | 1.7 |
| Completeness [%] | 98.5 | 96.7 |
| Redundancy | 3.3 | 3.1 |
| CC(1/2) | 0.999 | 0.647 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 5000, potassium chloride, magnesium chloride, PEG 400,Tris buffer |
