5LSP

107_A07 Fab in complex with fragment of the Met receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-05-29
Detector	PSI PILATUS 6M
Wavelength(s)	0.91376
Spacegroup name	P 2 21 21
Unit cell lengths	71.880, 82.279, 267.304
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.942 - 2.605
R-factor	0.2171
Rwork	0.215
R-free	0.25650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.999
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.950	48.950	2.740
High resolution limit [Å]	2.600	8.230	2.600
Rmerge	0.110	0.032	0.883
Number of reflections	49099
<I/σ(I)>	11.7	33.6	2
Completeness [%]	99.0	99	95.8
Redundancy	5.5	4.9	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	10% PEG 20,000, 20% PEG 550-MME, 0.1M Trizma/Bicine pH 8.5, 0.03M magnesium chloride, 0.03M calcium chloride