5LSO
Crystal structure of SPF45 UHM domain with cyclic peptide inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-22 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.600, 73.590, 45.010 |
| Unit cell angles | 90.00, 97.90, 90.00 |
Refinement procedure
| Resolution | 38.130 - 2.220 |
| R-factor | 0.1972 |
| Rwork | 0.181 |
| R-free | 0.24440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2peh |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.759 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.130 | 2.300 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.076 | 0.212 |
| Number of reflections | 11806 | |
| <I/σ(I)> | 14.61 | 5.29 |
| Completeness [%] | 98.0 | 88 |
| Redundancy | 5.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 50mM MES pH 6, 70% MPD |
