5LQF
CDK1/CyclinB1/CKS2 in complex with NU6102
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.989 |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.040, 67.750, 85.060 |
| Unit cell angles | 103.88, 90.89, 90.42 |
Refinement procedure
| Resolution | 65.700 - 2.060 |
| R-factor | 0.20037 |
| Rwork | 0.198 |
| R-free | 0.25385 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yc3 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.233 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.700 | 2.100 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.092 | 1.160 |
| Number of reflections | 84841 | |
| <I/σ(I)> | 6.2 | |
| Completeness [%] | 97.3 | 95.5 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 277 | 0.1M MES/IMIDAZOLE BUFFER (PH6.7), 6.5% MPD, 5% PEG4K, 10% PEG1K PROTEIN AT 10-12 MG/ML |
