5LQB

Complex structure of human IL2 mutant, Proleukin, with Fab fragment of NARA1 antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	201.757, 36.233, 88.707
Unit cell angles	90.00, 102.93, 90.00

Refinement procedure

Resolution	58.740 - 1.950
R-factor	0.19435
Rwork	0.189
R-free	0.29083
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ink 3tt1
RMSD bond length	0.023
RMSD bond angle	2.084
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.740	2.000
High resolution limit [Å]	1.950	1.950
Rmeas	0.079
Number of reflections	39422
<I/σ(I)>	14.2	2.59
Completeness [%]	84.8	96
Redundancy	3.2	3.22

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG3350 0.2M Sodium Nitrate