5LPU

Crystal structure of Annexin A2 complexed with S100A4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-06
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	71.740, 61.240, 148.160
Unit cell angles	90.00, 88.69, 90.00

Refinement procedure

Resolution	49.374 - 2.100
R-factor	0.1821
Rwork	0.181
R-free	0.20730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hyw
RMSD bond length	0.004
RMSD bond angle	0.683
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.374	2.170
High resolution limit [Å]	2.100	2.100
Number of reflections	75455
<I/σ(I)>	23.1	2.42
Completeness [%]	100.0	99.87
Redundancy	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.1M Bis Tris pH 6.5, 0.2M ammonium acetate 4% PEG4000 and 1,5% glycerol