5LP8

Crystal structure of an asymmetric dimer of the ubiquitin ligase HUWE1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-27
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 63
Unit cell lengths	177.464, 177.464, 106.259
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.150 - 2.700
R-factor	0.195
Rwork	0.194
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h1d
RMSD bond length	0.003
RMSD bond angle	0.743
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.150	2.780
High resolution limit [Å]	2.700	2.700
Rmerge	0.058	0.349
Number of reflections	52053
<I/σ(I)>	16.2	2.6
Completeness [%]	99.4	98.5
Redundancy	4.2	2.89

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Hepes pH 7, PEG20000