5LOB

Structure of the Ca2+-bound Rabphilin3A C2B- SNAP25 complex (C2 space group)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	153.970, 58.754, 125.311
Unit cell angles	90.00, 103.24, 90.00

Refinement procedure

Resolution	42.527 - 3.300
R-factor	0.2423
Rwork	0.240
R-free	0.28620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cm5
RMSD bond length	0.002
RMSD bond angle	0.377
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((dev_2341: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.420	3.480
High resolution limit [Å]	3.300	3.300
Rmerge	0.144
Number of reflections	12936
<I/σ(I)>	6	1.8
Completeness [%]	79.9	81.4
Redundancy	2.6	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	30% PEG3350 0.35 M Ammonium sulfate 0.1 M Tris pH 8.5