5LLU

Structure of the thermostabilized EAAT1 cryst-II mutant in complex with L-ASP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.009
Spacegroup name	P 63
Unit cell lengths	123.320, 123.320, 89.570
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 3.320
R-factor	0.2096
Rwork	0.207
R-free	0.25410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nwx
RMSD bond length	0.009
RMSD bond angle	1.050
Data reduction software	XDS (VERSION May 1, 2016 BUILT=20160517)
Data scaling software	XSCALE (VERSION May 1, 2016 BUILT=20160517)
Phasing software	PHASER (2.5.1)
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.870	3.850
High resolution limit [Å]	3.320	3.320
Rmerge	0.150	8.031
Number of reflections	11556
<I/σ(I)>	13
Completeness [%]	100.0	100
Redundancy	28.2	26.2
CC(1/2)	0.999	0.313

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	277	30% PEG400, 100 mM Tris pH 8.2, 50 mM Calcium chloride, 50 mM Barium chloride