5LIZ
The structure of Nt.BspD6I nicking endonuclease with all cysteines mutated by serine residues at 0.19 nm resolution .
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.96862 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.867, 93.059, 76.580 |
| Unit cell angles | 90.00, 112.89, 90.00 |
Refinement procedure
| Resolution | 19.550 - 1.930 |
| R-factor | 0.20863 |
| Rwork | 0.207 |
| R-free | 0.23927 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ewf |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.032 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.450 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.129 | 0.636 |
| Number of reflections | 57969 | |
| <I/σ(I)> | 7.1 | 1.9 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 16% PEG 8000 40MM POTASSIUM PHOSPHATE 20% GLYCEROL |
