5LIZ

The structure of Nt.BspD6I nicking endonuclease with all cysteines mutated by serine residues at 0.19 nm resolution .

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Nicking endonuclease N.BspD6I	polymer	610	71637.8	1	UniProt (Q8GCA3)	Bacillus sp.
2	B (A)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
3	C (A)	water	water		18.0	155	Chemie (HOH)

Sequence modifications

A: 1 - 604 (UniProt: Q8GCA3)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ser 11	Cys 11	engineered mutation
Ser 160	Cys 160	engineered mutation
Ser 508	Cys 508	engineered mutation
Ser 578	Cys 578	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	71637.8
Non-Polymers*	Number of molecules	1
	Total formula weight	95.0
All*	Total formula weight	71732.8

*Water molecules are not included.