5LGS

Crystal structure of mouse CARM1 in complex with ligand P2C3u

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 21 21 2
Unit cell lengths	74.986, 98.596, 208.172
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.971 - 2.100
R-factor	0.1877
Rwork	0.186
R-free	0.21470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ih3
RMSD bond length	0.002
RMSD bond angle	0.516
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((dev_2386: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.971	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.132	2.753
Number of reflections	90877
<I/σ(I)>	10.4	0.7
Completeness [%]	100.0	99.9
Redundancy	6.7	6.9
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Tris-HCl pH 8.5 100 mM PEG 3350 20 % A.S. 200 mM