5LGR

Crystal structure of mouse CARM1 in complex with ligand P1C3u

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 21 21 2
Unit cell lengths	74.831, 98.544, 208.336
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.048 - 2.000
R-factor	0.1941
Rwork	0.192
R-free	0.23370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ih3
RMSD bond length	0.002
RMSD bond angle	0.540
Data reduction software	XDS
Data scaling software	Aimless (0.5.25)
Refinement software	PHENIX (dev_2386)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.950	47.950	2.030
High resolution limit [Å]	2.000	10.950	2.000
Rmerge	0.174	0.033	3.226
Rmeas	0.189
Rpim	0.073
Total number of observations	700081
Number of reflections	104813
<I/σ(I)>	8
Completeness [%]	100.0	98.4	99.9
Redundancy	6.7	5.6	6.6
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	Tris-HCl pH 8.5 100 mM PEG 3350 14 % Ammonium Sulfate 200 mM