5LGP

Crystal structure of mouse CARM1 in complex with ligand P1C3s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 21 21 2
Unit cell lengths	74.794, 98.714, 207.253
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.000 - 2.040
R-factor	0.1889
Rwork	0.187
R-free	0.21930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ih3
RMSD bond length	0.006
RMSD bond angle	0.691
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((dev_2386: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	2.070
High resolution limit [Å]	2.040	2.040
Rmerge	0.148	2.331
Number of reflections	98498
<I/σ(I)>	9.7	0.8
Completeness [%]	100.0	100
Redundancy	6.6	6.4
CC(1/2)	0.998	0.313

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	100 mM Tris-HCl pH 8.5 16% PEG 3350 200 mM A.S.