5LGK
Crystal structure of the human IgE-Fc bound to its B cell receptor derCD23
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 |
| Unit cell lengths | 80.520, 85.320, 88.230 |
| Unit cell angles | 117.87, 90.32, 112.62 |
Refinement procedure
| Resolution | 75.960 - 3.500 |
| R-factor | 0.2665 |
| Rwork | 0.266 |
| R-free | 0.27840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ezm 2WQR and 4J6J |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.840 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.000 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.319 | 0.758 |
| Number of reflections | 21032 | |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 89.3 | |
| Redundancy | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 15% (w/v) PEG 8,000, 0.1 M sodium citrate, 0.05 M ammonium sulfate |
