5LBS
structural basis of Zika and Dengue virus potent antibody cross-neutralization
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 60.799, 121.353, 257.855 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.960 - 2.410 |
| R-factor | 0.1949 |
| Rwork | 0.194 |
| R-free | 0.21740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uta |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.460 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Number of reflections | 74842 | |
| <I/σ(I)> | 6.7 | 1.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.5 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 1.26M (NH4)2SO4 0.1M CHES pH 9.5 0.2M NaCl |
