5LB8
Crystal structure of human RECQL5 helicase APO form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979490 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.740, 68.740, 396.307 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.610 - 3.400 |
| R-factor | 0.3025 |
| Rwork | 0.301 |
| R-free | 0.32180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.610 | 3.670 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.117 | 1.139 |
| Number of reflections | 14135 | |
| <I/σ(I)> | 13.7 | 2.1 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 8.2 | 7.5 |
| CC(1/2) | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 17.5% Peg 3350, 0.2M Potassium Thiocyanate, 0.1M Hepes pH 7.0, 10% ethelene glycol |
