5LB3
Crystal structure of human RECQL5 helicase in complex with ADP/Mg.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976250 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.757, 62.943, 104.703 |
| Unit cell angles | 90.00, 95.44, 90.00 |
Refinement procedure
| Resolution | 19.880 - 1.800 |
| R-factor | 0.1968 |
| Rwork | 0.195 |
| R-free | 0.23350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cdg |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.796 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1682) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.880 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.078 | 0.812 |
| Number of reflections | 87336 | |
| <I/σ(I)> | 8.8 | 1.3 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 23% PEG6K, 0.15M lithium chloride, 2.5% ethylene glycol |
