5LAS

HIF prolyl hydroxylase 2 (PHD2-R281C/P317C/R396T) cross-linked to HIF-1alpha NODD-L397C/D412C and N-oxalylglycine (NOG) (complex-3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-26
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.83441
Spacegroup name	P 1 21 1
Unit cell lengths	43.579, 73.642, 70.146
Unit cell angles	90.00, 91.25, 90.00

Refinement procedure

Resolution	43.569 - 2.100
R-factor	0.187
Rwork	0.185
R-free	0.22120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hqr
RMSD bond length	0.004
RMSD bond angle	0.702
Data reduction software	HKL-2000 (706E)
Data scaling software	HKL-2000 (706E)
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.569	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.175	0.681
Number of reflections	26258
<I/σ(I)>	7.75	2.5
Completeness [%]	99.2	98.8
Redundancy	3.4	3.2
CC(1/2)	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.1 M citrate pH 5.0, 20 % w/v polyethylene glycol 6000