5LAS

HIF prolyl hydroxylase 2 (PHD2-R281C/P317C/R396T) cross-linked to HIF-1alpha NODD-L397C/D412C and N-oxalylglycine (NOG) (complex-3)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Egl nine homolog 1	polymer	252	27874.6	2	UniProt (Q9GZT9) Pfam (PF13640) In PDB	Homo sapiens (Human)	Hypoxia-inducible factor prolyl hydroxylase 2,HPH-2,Prolyl hydroxylase domain-containing protein 2,PHD2,SM-20
2	C, D	Hypoxia-inducible factor 1-alpha	polymer	19	1895.2	2	UniProt (Q16665) In PDB	Homo sapiens	HIF1-alpha,ARNT-interacting protein,Basic-helix-loop-helix-PAS protein MOP1,Class E basic helix-loop-helix protein 78,bHLHe78,Member of PAS protein 1,PAS domain-containing protein 8
3	A, B	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
4	A, B	N-OXALYLGLYCINE	non-polymer		147.1	2	Chemie (OGA)
5		water	water		18.0	205	Chemie (HOH)

Sequence modifications

A, B: 181 - 426 (UniProt: Q9GZT9)

PDB	External Database	Details
Gly 175	-	expression tag
Ser 176	-	expression tag
His 177	-	expression tag
Met 178	-	expression tag
Ala 179	-	expression tag
Ser 180	-	expression tag
Ala 201	Cys 201	engineered mutation
Cys 281	Arg 281	engineered mutation
Cys 317	Pro 317	engineered mutation
Thr 396	Arg 396	engineered mutation
Ala 398	Arg 398	engineered mutation

C, D: 395 - 413 (UniProt: Q16665)

PDB	External Database	Details
Cys 397	Leu 397	engineered mutation
Cys 412	Asp 412	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	59539.7
Non-Polymers*	Number of molecules	4
	Total formula weight	404.0
All*	Total formula weight	59943.8

*Water molecules are not included.