5L9B
HIF PROLYL HYDROXYLASE 2 (PHD2/ EGLN1) IN COMPLEX WITH 2-OXOGLUTARATE (2OG) AND HIF-1ALPHA CODD (556-574)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.193, 76.395, 70.962 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 40.193 - 1.950 |
| R-factor | 0.1607 |
| Rwork | 0.157 |
| R-free | 0.18650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hqr |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.610 |
| Data reduction software | HKL-2000 (706e) |
| Data scaling software | HKL-2000 (706e) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.193 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.167 | 0.900 |
| Number of reflections | 31425 | |
| <I/σ(I)> | 8.6543 | 1.713 |
| Completeness [%] | 99.1 | 98.2 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.2 M Magnesium chloride hexahydrate, 0.1 M BIS-TRIS pH 5.5, 25% w/v polyethylene glycol 3350 |
