5L1H

AMPA subtype ionotropic glutamate receptor GluA2 in complex with noncompetitive inhibitor GYKI53655

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	92.591, 110.636, 599.892
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.488 - 3.801
R-factor	0.2772
Rwork	0.276
R-free	0.29240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kg2
RMSD bond length	0.002
RMSD bond angle	0.531
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.488
High resolution limit [Å]	3.800	3.800
Rmerge	0.198	1.610
Number of reflections	115387
<I/σ(I)>	6.26
Completeness [%]	99.7
Redundancy	5.95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	11-14% (w/v) PEG 6,000, 0.1 M ammonium phosphate and 0.1 M TRIS (pH 7.9-8.0)