5L1G

AMPA subtype ionotropic glutamate receptor GluA2 in complex with GYKI-Br

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.92
Spacegroup name	P 21 21 21
Unit cell lengths	92.790, 110.400, 600.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.912 - 4.507
R-factor	0.2586
Rwork	0.257
R-free	0.29470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kg2
RMSD bond length	0.003
RMSD bond angle	0.576
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.912
High resolution limit [Å]	4.500
Number of reflections	69606
<I/σ(I)>	9.17
Completeness [%]	99.0
Redundancy	8.45

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	sodium acetate