5L0P
Symmetry-based assembly of a two-dimensional protein lattice
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-14 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 131.313, 54.833, 88.480 |
Unit cell angles | 90.00, 128.47, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
Rwork | 0.198 |
R-free | 0.23560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 1JI7 & 2FU4 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.350 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.283 | |
Number of reflections | 20782 | |
<I/σ(I)> | 15.9 | |
Completeness [%] | 93.9 | |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 100 mM Tris, pH 8.0, 3.5 M sodium chloride |