5L0H

Human metavinculin MVt cardiomyopathy-associated mutant R975W (residues 959-1134) in complex with PIP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 64 2 2
Unit cell lengths	80.951, 80.951, 131.762
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	70.110 - 2.900
R-factor	0.2559
Rwork	0.255
R-free	0.27780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3myi
RMSD bond length	0.010
RMSD bond angle	1.140
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	BUSTER (2.10.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.110	3.080
High resolution limit [Å]	2.900	2.900
Rpim	0.018
Number of reflections	6145
<I/σ(I)>	43.5	6.8
Completeness [%]	100.0	100
Redundancy	40.8	42.5
CC(1/2)	0.983	0.972

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	0.2 M LiSO4, 0.1 M HEPES (pH 7.5), and 22% PEG 3350