5L0D
Human Metavinculin(residues 959-1130) in complex with PIP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-11 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.617, 58.550, 112.492 |
| Unit cell angles | 90.00, 113.15, 90.00 |
Refinement procedure
| Resolution | 50.130 - 2.750 |
| R-factor | 0.2092 |
| Rwork | 0.207 |
| R-free | 0.25940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3myi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rpim | 0.048 | |
| Number of reflections | 19048 | |
| <I/σ(I)> | 16.3 | 8.2 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 8.7 | 8.6 |
| CC(1/2) | 0.995 | 0.987 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.4 | 294 | 20 mM citric acid, 80-100 mM Bis-Tris propane (pH 3.4), and 12-16% (w/v) PEG 3350 |
