5L04

STRUCTURE OF INTERFERON LAMBDA 1 RECEPTOR WITH HUMAN KINASE JAK1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-02
Detector	RAYONIX MX300HE
Wavelength(s)	1.00000
Spacegroup name	C 1 2 1
Unit cell lengths	165.700, 53.800, 87.390
Unit cell angles	90.00, 114.72, 90.00

Refinement procedure

Resolution	79.380 - 2.100
R-factor	0.1707
Rwork	0.169
R-free	0.23040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.852
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.1.1)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	99.000	99.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.077	0.050	0.634
Rmeas	0.081
Rpim	0.025
Total number of observations	384056
Number of reflections	40748
<I/σ(I)>	7.8
Completeness [%]	98.6	99.7	89.7
Redundancy	9.4	9.4	8
CC(1/2)		0.998	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	7 mg/ml of protein in 50 mM Hepes buffer (pH 7.5) with 150 mM NaCl, mixesd with 1:1 ratio with 18% (w/v) PEG 3350, 0.2 M amonium formate