5KXI

X-ray structure of the human Alpha4Beta2 nicotinic receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	93.15
Detector technology	PIXEL
Collection date	2016-02-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	127.099, 132.633, 202.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.996 - 3.941
R-factor	0.286
Rwork	0.285
R-free	0.30740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Homology model
RMSD bond length	0.003
RMSD bond angle	0.745
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	4.010
High resolution limit [Å]	3.940	3.940
Rmerge	0.137
Rpim	0.044	0.716
Number of reflections	30759
<I/σ(I)>	14.9	1.1
Completeness [%]	99.5	97.8
Redundancy	9.1	7.5
CC(1/2)		0.547

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287.15	0.05 M ADA pH 6.8, 12.5% PEG 1500 and 10% PEG 1000