5KX5

Crystal structure of tubulin-stathmin-TTL-Compound 11 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	104.790, 153.900, 185.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.470 - 2.500
R-factor	0.1996
Rwork	0.197
R-free	0.25140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4i55
RMSD bond length	0.010
RMSD bond angle	1.160
Data scaling software	SCALEPACK
Refinement software	BUSTER-TNT (2.11.6)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.470	100.000	2.540
High resolution limit [Å]	2.500	6.790	2.500
Rmerge	0.152	0.045
Number of reflections	101681
<I/σ(I)>	3.6
Completeness [%]	97.8	99.6	78.4
Redundancy	5.9	6.3	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	precipitant solution: 3% PEG 4K, 4-6% glycerol, 30 mM MgCl2, 30 mM CaCl2, 100 mM MES/Imidazole pH 6.7