5KW9

Structural Basis for Norovirus Neutralization by a HBGA Blocking Human IgA Antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	80
Detector technology	CCD
Collection date	2015-03-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.977
Spacegroup name	P 65 2 2
Unit cell lengths	162.000, 162.000, 146.810
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	81.000 - 2.300
R-factor	0.1876
Rwork	0.186
R-free	0.21230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zl5 4rqq
RMSD bond length	0.003
RMSD bond angle	0.678
Data reduction software	iMOSFLM
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.000	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.120	0.800
Number of reflections	50863
<I/σ(I)>	15.1	3
Completeness [%]	100.0	100
Redundancy	11.1	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2M Sodium Formate, 0.1M Bis Tris propane, pH 6.5 and 20% w/v PEG3350