5KT3

Teranry complex of human DNA polymerase iota(26-445) inserting dCMPNPP opposite template G in the presence of Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 65 2 2
Unit cell lengths	97.829, 97.829, 202.887
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.060 - 2.640
R-factor	0.2065
Rwork	0.204
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.024
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.060	50.000	2.690
High resolution limit [Å]	2.640	7.160	2.640
Rmerge	0.124	0.035	0.829
Number of reflections	17727
<I/σ(I)>	6.3
Completeness [%]	100.0	99.8	100
Redundancy	21.2	17.9	21.8
CC(1/2)		1.000	0.946

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277.15	18% PEG MME 5000, 0.1M MES buffer, 0.3M ammonium sulfate