5KSZ
hMiro EF hand and cGTPase domains in the GMPPCP-bound state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-19 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.978720 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 74.180, 74.180, 218.859 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.680 - 2.500 |
R-factor | 0.2134 |
Rwork | 0.211 |
R-free | 0.26570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4c0l |
RMSD bond length | 0.004 |
RMSD bond angle | 0.612 |
Data reduction software | MOSFLM (7.1.0) |
Data scaling software | SCALA (3.3.21) |
Phasing software | PHENIX (2.5.6) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.249 | |
Number of reflections | 22095 | |
<I/σ(I)> | 10.1 | |
Completeness [%] | 100.0 | |
Redundancy | 17.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 12 mg/mL protein, 6 mM magnesium chloride, 10 mM GMPPCP, 0.2 M ammonium sulfate, 0.1 M trisodium citrate, pH 5.6, 17.5% w/v PEG4000 |